FastPfu 快速高保真 DNA 聚合酶 -AP221

  產(chǎn)品名稱  

TransStart^? FastPfu DNA Polymerase(AP221)

  應(yīng)用專利  

專利號：ZL201510271941.3    ZL2023113204561    ZL2023118400050

  高分文獻(xiàn)集錦  

?Lei Z, Meng H, Liu L, et al. Mitochondrial base editor induces substantial nuclear off-target mutations[J]. Nature, 2022.(IF 69.50)

?Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 68.16)

?Zhang H, Zhu Y, Liu Z, et al. A volatile from the skin microbiota of flavivirus-infected hosts promotes mosquito attractiveness[J]. Cell, 2022.(IF 66.85)

?Wang Y, Zhang S, Yang X, et al. Mesoscale DNA feature in antibody-coding sequence facilitates somatic hypermutation[J]. Cell, 2023.(IF 64.50)

?Lin Q, Jin S, Zong Y, et al. High-efficiency prime editing with optimized, paired pegRNAs in plants[J]. Nature Biotechnology, 2021.(IF 54.90)

?Zong Y, Liu Y, Xue C, et al. An engineered prime editor with enhanced editing efficiency in plants[J]. Nature Biotechnology, 2022.(IF 54.00)

?Liu J L, Yan X Q, Wu H, et al. RNA codon expansion via programmable pseudouridine editing and decoding[J]. Nature, 2025.(IF 48.50)

?Yang J, Zhao T, Fan J, et al. Structure-guided discovery of bile acid derivatives for treating liver diseases without causing itch[J]. Cell, 2024.(IF 45.60)

?Jin S, Lin Q, Luo Y, et al. Genome-wide specificity of prime editors in plants[J]. Nature Biotechnology, 2021.(IF 36.55)

?Wang S, Zong Y, Lin Q, et al. Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC–Cas9[J]. Nature biotechnology, 2020.(IF 36.55)

?Li C, Zhang R, Meng X, et al. Targeted, random mutagenesis of plant genes with dual cytosine and adenine base editors[J]. Nature biotechnology, 2020.(IF 35.72)

?Lin Q, Zong Y, Xue C, et al. Prime genome editing in rice and wheat[J]. Nature biotechnology, 2020.(IF 31.90)

?Song B, Chen Y, Liu X, et al. Ordered assembly of the cytosolic RNA-sensing MDA5-MAVS signaling complex via binding to unanchored K63-linked poly-ubiquitin chains[J]. Immunity, 2021.(IF 31.75)

?Li Y, Zhao L, Zhang Y, et al. Structural basis for product specificities of MLL family methyltransferases[J]. Molecular Cell, 2022.(IF 19.33)

?Li C, Zong Y, Jin S, et al. SWISS: multiplexed orthogonal genome editing in plants with a Cas9 nickase and engineered CRISPR RNA scaffolds[J]. Genome biology, 2020.(IF 18.36)

?Zhang H, Li Z, Zhou S, et al. A fungal NRPS-PKS enzyme catalyses the formation of the flavonoid naringenin[J]. Nature Communications, 2022.(IF 17.69)

?Lei Y, Fei P, Song B, et al. A loosened gating mechanism of RIG-I leads to autoimmune disorders[J]. Nucleic acids research, 2022.(IF 16.97)

?Fan J, Ran H, Wei P L, et al. An ortho‐Quinone Methide Mediates Disulfide Migration in the Biosynthesis of Epidithiodiketopiperazines[J]. Angewandte Chemie International Edition, 2023.(IF 16.82)

?Song Z, Zhou S, Zhang H, et al. Fungal secondary metabolism is governed by an RNA-binding protein CsdA/RsdA complex[J]. Nature Communications, 2023.(IF 16.60)

?Fan J, Ran H, Wei P L, et al. Pretrichodermamide A Biosynthesis Reveals the Hidden Diversity of Epidithiodiketopiperazines[J]. Angewandte  Chemie, 2023.(IF 16.82)

?Jin S, Fei H, Zhu Z, et al. Rationally designed APOBEC3B cytosine base editors with improved specificity[J]. Molecular cell, 2020.(IF 15.58)

?Lin R, Liang J, Wang R, et al. The raphe dopamine system controls the expression of incentive memory[J]. Neuron, 2020.(IF 14.40)

  產(chǎn)品特點  

? 快速：4 kb/min 的延伸速度。

? 高保真性：保真性為普通 Taq 酶的 54 倍，普通 Pfu 酶的 3 倍。

? 長片段擴(kuò)增：基因組 DNA 片段的擴(kuò)增可達(dá) 15 kb，Plasmid DNA 片段的擴(kuò)增可達(dá) 20 kb。

? 高特異性：采用“TransStart”雙封閉法新型熱啟動技術(shù)，同時封閉引物和模板。

? 擴(kuò)增能力強(qiáng)：獨有的 PCR Stimulant，提升該酶對復(fù)雜模板的擴(kuò)增能力。

? 擴(kuò)增產(chǎn)物為平端，可直接克隆于 pEASY^? -Blunt 系列載體中。 

  數(shù)據(jù)展示  

擴(kuò)增能力強(qiáng)

  產(chǎn)品信息